Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable
نویسندگان
چکیده
منابع مشابه
Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable.
The synthesis rates and half-lives of the individual mitochondrial ribosomal ribonucleic acid (RNA) and polyadenylic acid-containing RNA species in HeLa cells have been determined by analyzing their kinetics of labeling with [5-3H]-uridine and the changes in specific activity of the mitochondrial nucleotide precursor pools. In one experiment, a novel method for determining the nucleotide precur...
متن کاملLocalization of the polyadenylate sequences in messenger ribonucleic acid and in the heterogeneous nuclear ribonucleic acid of HeLa cells.
Poly(A) sequences are covalently bound to the 3’ termini of mRNA and many heterogeneous nuclear RNA molecules of HeLa cells. Localization of the poly(A) sequences has been established in these experiments by the observation that a large fraction of the label introduced in the oxidized 2’,3’cis-glycol groups of these RNAs by sodium borohydride reduction, is recovered in the poly(A) sequences rel...
متن کاملRibonucleic Acid and Protein Synthesis in Mitotic Hela Cells
HeLa cells arrested in mitosis were obtained in large numbers, with only very slight interphase cell contamination, by employing the agitation method of Terasima and Tolmach, and Robbins and Marcus. Protein synthesis and RNA synthesis were almost completely suppressed in mitotic cells. Active polyribosomes were nearly absent in mitotic cells as compared with interphase cells treated in the same...
متن کاملRibonucleic acid synthesis in Streptomyces antibioticus: stable ribonucleic acid species synthesized by young and old cells.
In studies of RNA synthesis by intact cells and cell-free extracts of Streptomyces antibioticus, it has been found that 48 hr cells (producing actinomycin) and cell-free extracts are less efficient than 12 hr cells (not producing actinomycin) and extracts in the synthesis of RNA. Analysis of the products of "in viva" and "in vitro" RNA synthesis by sucrose gradient cent trifugation reveals that...
متن کاملInhibitors of ribonucleic acid synthesis in Saccharomyces cerevisiae: decay rate of messenger ribonucleic acid.
Daunomycin and ethidium bromide, two deoxyribonucleic acid-intercalating drugs, inhibit ribonucleic acid (RNA) and protein synthesis in Saccharomyces cerevisiae. Both agents rapidly curtail uptake of radioactive adenine, whereas the kinetics of radioactive leucine uptake after drug addition are consistent with translation of a pool of exponentially decaying messenger RNA. Messenger RNA half-lif...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1981
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.1.6.497-511.1981